拟南芥ERD15基因缺失突变体的分子鉴定和表型分析

Molecular Identification and Phenotype of Arabidopsis thaliana Mutant erd15

摘要:

为探究ERD15基因功能，利用反向遗传学，通过PCR及半定量PCR筛选鉴定出拟南芥（Arabidopsis thaliana）ERD15基因的T-DNA插入纯合突变体，并对其表型进行观察分析。结果表明，erd15突变体莲座叶数目显著增多，提前3~4 d开花，突变体比野生型更早从营养生长转向生殖生长。拟南芥野生型植株主茎为圆柱体，平均直径1.29 mm，而erd15突变体主茎扁平，平均直径达到2.27 mm，具极显著差异。与野生型相比，erd15突变体果实心皮发育受到影响，隔膜上排列有多排种子，果荚顶端膨大，长度缩短37.67%，但角果平均结籽数升高。因此，ERD15基因参与了调控拟南芥植株的生殖生长过程。

Abstract:

In order to explore the function of ERD15 gene by reverse genetics, a homozygous T-DNA insertion mutant of ERD15 gene of Arabidopsis thaliana was identified by PCR and semi-quantitative PCR. The phenotype of erd15 mutant was observed. The results showed that the number of rosette leaves in erd15 mutant increased significantly and flowering time was 3-4 days earlier, indicating that the erd15 mutant from vegetative to reproductive growth was earlier than the wild type. The main stem of wild-type A. thaliana was cylindrical with an average diameter of 1.29 mm, while that of erd15 mutant became flattened with an average diameter of 2.27 mm. Compared with the wild type, the carpel development of erd15 mutant fruit was affected, multiple rows of seed were arranged on the septum, and the length of silique was shortened by 37.67% with swelled apex, but the average seed number of silique increased in erd15 mutant. Therefore, it was suggested that ERD15 gene was involved in regulating the reproductive growth of A. thaliana.